Stat5 y694

Author: plhg

August undefined, 2024

Web(C) Western blot analysis of phospho-STAT5 Y694 and STAT5 in STAT5B (N642H) transformed Ba/F3 cells after treatment with baricitinib, decernotinib, peficitinib, ruxolitinib, tofacitinib, and upadacitinib 2 μM or DMSO for 90 minutes. Table 1: WebImmunogen: Phosphorylated Human Phosphorylated Stat5 Peptide Application: Western blot (Routinely Tested) Target Molecular Weight: 92 kDa Concentration: 250 µg/ml RRID: AB_399386 Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide. Regulatory Status: RUO View All Formats Preparation And Storage

Anti-STAT5 (phospho Y694) antibody (ab98338) Abcam

WebSTAT5 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, translocates to the nucleus and activates transcription of specific genes. STAT5 is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation and survival. WebSTAT5 is a transcriptional activator which is also involved in intracellular signaling. Activation of STAT5 occurs upon phosphorylation at Tyr694. In some cancers STAT5 is … food store fruit shelves

Recombinant APC Anti-STAT5 (phospho Y694) antibody [Stat5Y694 ... - Abcam

WebNov 22, 2013 · As STAT5 Y694 phosphorylation depends on BCR-ABL kinase activity, we investigated whether phosphorylation of STAT5 S725 and STAT5 S779 requires BCR-ABL … WebMay 14, 2024 · STAT5 phospho-flow cytometry Flow cytometry was performed with Alexa Fluor 647 mouse anti-pSTAT5 Y694 (clone 47, BD Biosciences). MOLM-13 cells were cultured in CM for 4 h prior to... WebPhospho-STAT5 alpha (Tyr694) Antibody (71-6900) in ICC/IF Immunofluorescent analysis of STAT5 (pY694) was done on 70% confluent log phase HeLa cells; untreated & treated (serum starved for 16 hours followed by treatment with 20 ng/mL IFN alpha for 1 hour). foods to reduce uric acid naturally

Stat5 y694

An optimized multiplex flow cytometry protocol for the analysis of ...

WebDescription: This SRBCZX monoclonal antibody recognizes signal transducer and activator of transcription 5 (STAT5) when phosphorylated on tyrosine 694. STAT proteins are … WebChromatin immunoprecipitation analysis of Phospho-STAT5 alpha (pTyr694) was performed using cross-linked chromatin from 1 x 10 6 HCT 116 (Human colorectal carcinoma cell line) ... All lanes : Anti-STAT5a …

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WebSTAT5 inhibitors. Cell lines or CD34 cells from CML patients treated with pimozide revealed decreased pSTAT5 levels. 10 Furthermore, pimozide showed major effects on cell survival … WebDec 13, 2024 · STAT3-JAK2 expression resulted in phosphorylation of the residues within the fusion corresponding to JAK2 Y1007/1008 and STAT3 Y705 and phosphorylation of native STAT3 Y705 and STAT5 Y694.

WebFlow cytometric analysis of Stat5 (pY694) in human lysed whole blood. Whole blood was either left untreated (dashed line histogram) or treated with 100 ng/mL Recombinant … WebDec 6, 2024 · IL-2, a cytokine known to activate STAT5 , did induce phospho-STAT5 Y694 but at relatively low levels compared to TNF-Th17 cells. Interestingly, IL-2–treated TNFR2-deficient cells had even higher levels of phospho-STAT5 Y694 than WT T cells. Thus, signaling through TNFR2 positively regulates STAT5 activity, whose inhibition impairs the ...

WebPhospho-STAT5 (Tyr694) Antibody (11-9010-42) in Flow Intracellular staining of untreated (orange histogram) or 15-minute Human GM-CSF Recombinant Protein-treated (purple … WebMar 1, 2024 · PTP1B Deletion Promotes STAT5-Dependent T-cell Expansion, Activation, and Antitumor Immunity To elucidate how PTP1B deficiency promotes T cell–mediated antitumor immunity, we first assessed whether PTP1B deletion might enhance the αβ TCR signaling that is essential for T-cell activation and function ( 37 ).

WebThe presence of an SH2 domain within Stat5 suggests that it may directly interact with protein tyrosine kinases (PTKs) such as JAK2. The 47 monoclonal antibody recognizes the phosphorylated Y694 of Stat5a. The homologous phosphorylation site in Stat5b is Y699. Flow cytometric analysis of Stat5 (pY694). TF-1 cells

Web丁香通为您提供RecombinantH商品详情介绍：价格：￥3159，货号：CLPRO1153-2，品牌：Cedarlane，产地：请咨询，详见丁香通RecombinantH商品详情页； foods to reduce weight naturallyWebJun 4, 2024 · Subsequently, the activated kinases phosphorylate downstream effector molecules, including STAT5. Upon phosphorylation of tyrosine residue Y694, STAT5 homodimerizes and translocates to the nucleus where it activates the expression of STAT5 target genes, which are known to be involved in cell survival and cell cycle progression. 8 foods to reduce tremorsWebMar 24, 2024 · Genes modulated by Snhg1 and Vps13D in T cells mainly involve in membrane receptor-associated immune processes. a The gp33 activated P14 CD8 T cells from CD45.1 mice were enriched and transducted... foods to reduce uric acid in bodyWebDec 22, 2016 · Induction of STAT5 phosphorylation at Y694 (P-STAT5) diminished NPM1 expression, whereas inhibition of STAT5 phosphorylation enhanced NPM1 expression. Conversely, NPM1 not only negatively regulated STAT5 phosphorylation but also preserved unphosphorylated STAT5 level. foods to reduce potassiumWebStat5 is activated in response to a wide variety of ligands, including IL-2, GM-CSF, growth hormone, and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). … foods to reduce uric acid in your bodyWebAnti-STAT5 (phospho Y694) antibody See all STAT5 primary antibodies Description Rabbit polyclonal to STAT5 (phospho Y694) Host species Rabbit Tested applications Suitable … foods to reduce stomach gasWebAdd 10 µL of 500 nM Eu-labeled anti-STAT5 (Y694/Y699) Antibody and 100 μL of 500 nM ULight-labeled anti-STAT5 Antibody to 2390 µL of LANCE Detection Buffer 3) In a white Optiplate (384 wells): *In order to reduce evaporation, we recommend covering the OptiPlate with TopSeal-A PLUS during the incubation. ... food store gift cards