WebJul 22, 2024 · For reagentless detection, we employ a strand displacement strategy where fluorophore-conjugated aptamers are hybridized with a DNA competitor strand conjugated to a quencher molecule. The assay is utilized for rapid (2 min) measurement of glucose, adenosine triphosphate, l -tyrosinamide, and thrombin ex vivo. WebApr 3, 2024 · The ability to sense biological inputs using self-contained devices unreliant on external reagents or reporters would open countless opportunities to collect information about our health and environment. Currently, a very limited set of molecular inputs can be detected using this type of sensor format. The development of versatile reagentless …
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WebJan 11, 2006 · Reagentless, reusable, ultrasensitive electrochemical molecular beacon aptasensor J Am Chem Soc. 2006 Jan 11;128(1):117-24. doi: 10.1021/ja053121d. Authors … WebAs experts in the field of flow chemistry, Syrris understands the growing need for synthetic chemists to tackle the environmental impact of waste and the costs associated with its … list network shares powershell
Reagentless, Structure-Switching, Electrochemical Aptamer-Based …
WebNov 15, 2013 · In this paper, we describe a label-free and reagentless miRNA sensor based on an interpenetrated network of carbon nanotubes and electroactive polymer. The nanostructured polymer film presents very well-defined electroactivity in neutral aqueous medium from the quinone group embedded in the polymer backbone. WebDec 1, 2005 · Thrombin binding stabilizes the alternative G-quadruplex conformation of the aptamer, liberating the methylene blue (MB)-tagged oligonucleotide to produce a flexible, single-stranded DNA element. This allows the MB tag to collide with the gold electrode surface, producing a readily detectable Faradaic current at thrombin concentrations as … WebNov 1, 2024 · With this strategy, we successfully fabricated an electrochemical sensor on gold electrode for rapid, reagentless, and sensitive detection of aflatoxin B1 (AFB1), an important mycotoxin causing great health risks, by using a 26-mer DNA aptamer with MB on an internal T site (e.g., 18th T) and a thiol moiety at 5' terminal. list networks windows