Ovarian granulosa cell lines
WebJul 27, 2024 · Cell cultures. The human ovarian granulosa cell lines KGN and COV434 with STR (short tandem repeat) identification were cultured in DMEM (HyClone, Logan, UT, USA) containing 10% fetal bovine serum ... WebApr 10, 2024 · Furthermore, both APLN-13 and -17 improved the total antioxidant capacity of the ovarian granulosa cells, but such effects were not seen when the cells were …
Ovarian granulosa cell lines
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WebA granulosa cell or follicular cell is a somatic cell of the sex cord that is closely associated with the developing female gamete ... Embryology of ovarian granulosa cells ... Mural granulosa cells line the follicular wall and surround the fluid-filled antrum. The oocyte secretes factors that determine the functional differences between CCs ... WebApr 12, 2024 · This study investigated the roles of adiponectin in IVF treatment during Phase I (the basal stage before gonadotropin administration), Phase II (approximately 8 days …
WebGranulosa cell tumors (GCTs) are a type of ovarian tumor. They often produce hormones and lead to high estrogen levels. Women with a GCT may have irregular periods or bleeding after menopause. In young girls, a GCT may cause early puberty. When healthcare providers diagnose a GCT early, the outlook is usually good. Symptoms and Causes WebJul 4, 2024 · To further explore the effects of iron overload with EMFF on human ovarian granulosa cell function, we intervened KGN, a human ovarian granulosa cell line, with EMFF and follicular fluid in the ...
WebFeb 18, 2024 · Cell lines and cell culture. The human ovarian granulosa cell lines KGN and COV434 kept in our laboratory with STR (short tandem repeat) identification were cultured in DMEM (HyClone, USA) supplemented with 1% fetal bovine serum (Gibco, USA) and 100 IU/mL penicillin–streptomycin at 37 °C in a humidified atmosphere of 5% CO 2 … WebNational Center for Biotechnology Information
WebDerived from sampling site: Ovary; ovarian follicle; granulosa cell layer. Sequence variations: Mutation; HGNC; 1092; FOXL2; Simple; p.Cys134Trp (c.402C>G); ...
WebApr 11, 2024 · Key molecules of the Hippo signaling pathway, including MST1/2, YAP, and TAZ, play critical regulatory roles in ovarian granulosa cells [60,61,62] and can … newham newshareWebFeb 21, 2024 · The ovarian somatic cells further differentiate into several lineages, including the granulosa cells which surround and support developing oocytes. Proper … interview dishwasher olive garden redditGranulosa cell tumors (GCTs) are a type of ovarian tumor. They often produce hormones and lead to high estrogen levels. Women with a GCT may have irregular periods or bleeding after menopause. In young girls, a GCT may cause early puberty. When healthcare providers diagnose a GCT early, the outlook is … See more Experts don’t entirely understand what causes granulosa cell tumors. They often have a mutation (change) in the FOXL2 gene. FOXL2typically functions to help … See more The most common symptom of a GCT is high estrogen. In girls, high estrogen may cause early puberty. In adults, GCTs commonly cause: 1. Abnormal uterine … See more Without treatment, granulosa cell tumors can increase your risk of other health complications, including: 1. Endometrial hyperplasia, an unusually thick … See more newham nelftWebDec 22, 2024 · To trace the distinct cell lineages and characterize the gene expression dynamics of ovarian cells during the crucial period of ovarian development lasting from late fetal and early postnatal age, ovaries were collected from E16.5, postnatal day 0 (PD0) and 3 (PD3) mice and subjected to immunohistochemical staining and scRNA-seq analyses. newham news liveWebApr 11, 2024 · Culture of KGN granulosa cell line. KGN cell line was purchased from MeiSenCTCC company (human KGN cell line, CTCC-003-0105) and maintained in DMEM/F12 medium supplemented with 10% fetal bovine serum (FBS, Gibco, 10,099–141). KGN cell line were cultured at 37 °C in an incubator with 5% CO 2. Establishment of the … newham network telecare serviceWebSep 1, 2013 · Both cell lines have normal female karyotypes (46,XX) and were grown on irradiated mouse embryonic fibroblast feeders in primate embryonic stem medium (ReproCELL) that was supplemented with 4 ng/mL of basic fibroblast growth factor (bFGF; Invitrogen). ... These hESC-derived ovarian granulosa-like cells retained many … newham newsWebEnter the email address you signed up with and we'll email you a reset link. interview digital transformation aprilpost