Gapdh molecular weight 36
WebMar 21, 2024 · GAPDH: GAPDH or G3PDH is an acronym for glyceraldehyde-3-phosphate dehydrogenase. GAPDH is an enzyme in the glycolysis reaction and is widely distributed in cells in various tissues. ... β-Actin：β-actin is composed of 375amino acids and its molecular weight is about 42-43kDa. As an internal reference, β-actin is recognized as … WebMar 20, 2024 · GAPDH is a housekeeping gene and used as controls in both Western blot and qPCR. ... a splice variant with an extra exon with the molecular weight of 150 kDa is also expressed. Nuclear proteins - nuclear loading controls ... Human lamin B1 shares 95% identity with rodent homologs, and 36% identity with the fruit fly counterpart. Human …
Gapdh molecular weight 36
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WebGene target information for GAPDH - glyceraldehyde-3-phosphate dehydrogenase (human). Find diseases associated with this biological target and compounds tested … WebHowever, Idiomarina loihiensis enzyme sub-unit had an estimated molecular weight of approximately 36 kDa, somewhat higher than the one reported for the GAPDH subunit of different organisms like bacterial species Bacillus cereus (35 kDa) and the same weight of the amphibian Xenopus sp. (36 kDa) [23,24].
WebKey features and details Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control Suitable for: WB, IHC-P Reacts with: Mouse, Rat, Chicken, Cow, Human, Pig, Xenopus … WebThis antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH …
WebAug 21, 2007 · The apparent molecular weight of the purified native enzyme was 153 kDa. The protein is an homotetramer, composed of identical subunits with an apparent molecular weight of approximately 36 kDa. The Michaelis constants K m for both NAD + and d-G3P were 66 μM and 320 μM, respectively. WebFeb 1, 2012 · GAPDH has also been found to function in additional cellular process, such as transcription, apoptosis, oxidative stress and ER to Golgi transport. Application GAPDH …
WebFeb 10, 2024 · The objective of the current study was to examine the molecular targets underlying the anti-inflammatory effects of EGCG in activated microglia cells. ... 1× fluorescent molecular weight markers, and 40 mM dithiothreitol. Samples were heated at 95 °C for 5 min. The primary antibody dilution factors used were: mTOR 1:150, Akt3 …
WebGAPDH is a ubiquitously expressed and has a molecular mass of 36 kD. Though differentially expressed from tissue to tissue, GAPDH is frequently used as a loading … ohio will and testament templateWebAnti-GAPDH, Cat. No. AB2302, is a chicken polyclonal antibody that detects Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and is tested for use in Western Blotting. Anti-GAPDH Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. SDS CoA References ohio windsWebThe gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is mapped to human chromosome 12p13. 1] The protein localizes in the cytoplasm but can be translocated to the nucleus depending on cellular conditions. 2] Specificity Monoclonal Anti-GAPDH recognizes human, monkey, bovine, canine, rat, mouse, hamster, mink, rabbit, chicken, and turkey … myhumana account and settingsWebThe molecular weight of GAPDH is 36 kD. What is GAPDH? GAPDH stands for glyceraldehyde-3-phosphate dehydrogenase and is often referred to as a "housekeeping" protein. It is recognized as an important enzyme involved in metabolic pathways, aiding … Calculated molecular weight: 375 aa, 42 kDa: Observed molecular weight: 42 … myhumana account pharmacyGlyceraldehyde 3-phosphate dehydrogenase (abbreviated GAPDH) (EC 1.2.1.12) is an enzyme of about 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activatio… ohio wine and mixed beverage taxWebJan 23, 2007 · Oxidative stress can promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process called nucleocytoplasmic coagulation. ... including oxidation of Tyr-45 and Met-105. This cascade of oxidations may augment GAPDH misfolding, leading to intermolecular disulfide cross-linking and ... 36 … ohio windstreamWebThe molecular docking results of Rh4 and HDAC2 indicate a binding energy of −6.06 kcal/mol, suggesting the potential of Rh4 as a targeting modulator of HDAC2. Mechanistically, Rh4 induces apoptosis of breast cancer cells by the HDAC2-mediated caspase pathway and inhibits the HDAC2-mediated JAK/STAT pathway to regulate the … myhumana account register