Web1 Mar 1999 · For transformations with PCR products, we used 80 μl of the PCR mixture per transformation. For transformations with linearized plasmids or restriction fragments, we used 2 to 10 μg of digested DNA per transformation. For disruption of ENX3, we used a Frozen-EZ Yeast Transformation II kit (Zymo Research) and followed the supplier’s ... WebFrozen-EZ Yeast Transformation II T2001 Highlights ♦ High transformation efficiency that yields approximately 10 5-10 6 transformants per µg plasmid DNA (circular). ♦ Frozen …
Frozen-EZ Yeast Transformation II Kit - ZYMO RESEARCH
Web29 Apr 2024 · Yeast extract Peptone Dextrose (YPD) medium: Dissolve 20 g peptone and 10 g yeast extract in 950 mL ddH 2 O and autoclave. Separately, dissolve 20 g dextrose in 50 mL ddH 2 O and sterilize by filtration. Combine and allow to cool before use. 3. YPD plates: Dissolve 20 g peptone, 10 g yeast extract, and 15 g agar in 950 mL ddH 2 O and … Web14 Apr 2024 · Commercial transformation kit like the Frozen-EZ Yeast Transformation II Kit™ (Zymoresearch). 3. The two plasmids encoding the complete TALEN sequences. 4. If necessary recombination matrix prepared at 500 ng/μL. 5. Rich medium YPD: 10 g/L yeast extract, 10 g/L bactopeptone, 10 g/L glucose. 6. memphis eucharistic congress
Frozen-EZ Yeast Transformation II™ Kit (120 Rxns)
WebFrozen-EZ Solution 1 - 60 ml - T2002 from Zymo Research. Frozen-EZ Solution 1 - 60 ml - T2002 from Zymo Research. Tel: +44 (0)1223 316 855; register; ... Frozen-EZ Yeast Transformation II™ Kit T2001 · Zymo Research: T2001. Zymo Research: 120 reactions: £156.00. 120 reactions. view : Frozen-EZ Solution 2 T2003 · Zymo Research: T2003. … WebThe Frozen-EZ Yeast Transformation II Kit is designed to make yeast transformations and library screening easier and more efficient than currently available methods. The … WebFrozen-EZ Yeast Transformation II Kit (Zymo Research) and select transformed cells on complete supplement mixture lacking leucine and tryptophan (CSM ‑Leu ‑Trp) at 30 °C for 3–4 days. memphis esthetician school