WebJan 26, 2024 · A luciferase reporter assay was performed according to the manufacturer’s instructions. ChIRP Lnc-17-92 and LacZ antisense DNA probes were designed using the online probe designer at singlemoleculefish.com. Oligonucleotides were biotinylated at the 3' end with an 18-carbon spacer arm. WebAug 11, 2024 · CHIRP assay was performed according to previous research 55. ILCPs were cross-linked with 1% glutaraldehyde at room temperature for 10 min and quenched by 0.1 M glycine for 5 min. Then cells were washed twice with PBS, lysed with Lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris, supplemented with PMSF, proteinase inhibitor cocktails, and …
Microenvironment-regulated lncRNA-HAL is able to promote ... - PubMed
WebJan 1, 2024 · The applications of ChIRP. ChIRP has proven to be a highly efficient and powerful tool for the functional analysis of lncRNAs, thus serving as a useful method to … WebFeb 6, 2024 · ChIP assays were performed using the Pierce Magnetic ChIP Kit (Thermo Fisher Scientific). Briefly, cells were crosslinked with 1% formaldehyde for 10 min at room temperature. Glycine was then added and incubated with the cells for 5 min to quench the crosslinking reaction. photographers similar to cindy sherman
Chromatin Isolation by RNA Purification (ChIRP) Protocol
WebAug 29, 2024 · Additionally, ChIRP assays reveal that LAIR binds 5′ and 3′ UTR genomic regions of the LRK1 gene. These results imply that LAIR overexpression increases rice yield and regulates the expression... WebThe EZ-Magna ChIRP RNA Interactome Kit allows analysis, and mapping of regions of chromatin interacting with chromatin-associated RNAs. Based on the ChIRP method … WebDec 23, 2024 · ( A) Co-IP assay with antibody (Ab) against PTBP1 (top) or PSF (bottom) detecting the PTBP1/PSF interaction within whole-cell extracts of HEK293 cells and the PTBP1/PSF interaction after RNase T1 or … how does webauthn work